Caspase检测试剂盒-细胞生物学检测-试剂-生物在线
上海博升生物科技有限公司
Caspase检测试剂盒

Caspase检测试剂盒

商家询价

产品名称： Caspase检测试剂盒

英文名称： Caspase

产品编号： FAM100/800

产品价格： 0

产品产地： 美国

品牌商标： Cell Tech

更新时间： null

使用范围： null

上海博升生物科技有限公司
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进入展台

Key Benefits:
  • Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
  • Cell permeablity permits direct visualization of cytosolic apoptotic events.
  • Apoptotic cell population does not diminish over time.
  • Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
  • Works in diverse cell lines: human, rodent, Drosophila.
  • Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
  • Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
  • Works with fluorescence microscopy, 96-well fluorescence plate readers, or flow cytometry.
  • Yields both quantitative and qualitative results. Gives strong signal with little background noise.
  • Mark activity across the range of caspase proteins. Poly-caspase and caspase-specific assays to target caspases 1, 2, 3, 6, 8, 9, or 10 are available.
  • Applications - Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscope
Assay Principle

APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems