pMSCV-TAP
(Plasmid #12570)

PURPOSE

(Empty Backbone)
DEPOSITING LAB

David Sabatini
PUBLICATION

Frias et al Curr Biol. 2006 Aug 15. ():.
PRICE

$65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pMSCV-TAP
(Search Vector Database)
Backbone manufacturer
Modified from Clontechs pMSCV
Backbone size (bp)6800
Vector type
Mammalian Expression, Retroviral
Selectable markers
Puromycin

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

Gene/Insert name
None
Tag / Fusion Protein
- TAP (N terminal on backbone)

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ sequencing primerMSCV primer

(Common Sequencing Primers)

RESOURCE INFORMATION

A portion of this plasmid was derived from a plasmid made by
TAP was PCRd from pBSactTAP, a gift from Dr. Matthias Wilm (Heidelberg, Germany)
Terms and Licenses
- UBMTA

DEPOSITOR COMMENTS

A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5 -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3 and right primer 5 -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3 . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See authors map for more information (Note that the NotI site is not precisely underlined in the authors map).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pMSCV-TAP was a gift from David Sabatini (Addgene plasmid # 12570)
For your References section:

mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001PubMed 16919458

pMSCV-TAP逆病毒载体质粒图谱序列抗性

pMSCV-TAP (Plasmid #12570)