兔单克隆抗体Cleaved Caspase-3 (Asp175) (5A1E)-抗体-抗体-生物在线
兔单克隆抗体Cleaved Caspase-3 (Asp175) (5A1E)

兔单克隆抗体Cleaved Caspase-3 (Asp175) (5A1E)

商家询价

产品名称： 兔单克隆抗体Cleaved Caspase-3 (Asp175) (5A1E)

英文名称： Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb

产品编号： 9664S

产品价格： null

产品产地： usa

品牌商标： cst

更新时间： null

使用范围：

济南翔宇生物科技有限公司
  • 联系人 :
  • 地址 : 济南市天桥区
  • 邮编 : 2500
  • 所在区域 : 山东
  • 电话 : 187****5463
  • 传真 : 18754115463
  • 邮箱 : 1024837510@qq.com
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Product Usage Information

Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry 1:2000
Immunofluorescence 1:400
Flow Cytometry 1:6400


Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

 

 

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.


Species Reactivity: Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology: Dog

 

 

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).


1.  Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.

2.  Nicholson, D.W. et al. (1995) Nature 376, 37-43.


Entrez-Gene Id 836
Swiss-Prot Acc. P42574
 

Data Sheets & Documentation