DCK polyclonal antibody
产品名称： DCK polyclonal antibody
英文名称： DCK polyclonal antibody
产品编号： PAB2020
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DCK.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DCK.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunoprecipitation (1:500-1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity.
Hartford CM, Duan S, Delaney SM, Mi S, Kistner EO, Lamba JK, Huang RS, Dolan ME.Blood. 2009 Mar 5;113(10):2145-53. Epub 2008 Dec 24.
- 2.
- Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Lamba JK, Crews K, Pounds S, Schuetz EG, Gresham J, Gandhi V, Plunkett W, Rubnitz J, Ribeiro R.J Pharmacol Exp Ther. 2007 Dec;323(3):935-45. Epub 2007 Sep 12.
- Applications
- Western Blot (Tissue lysate)
- The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.
- Immunoprecipitation
- Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with 7.5 ug (microgram) of DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21.
Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1 : 700 dilution of DCK polyclonal antibody (Cat # PAB2020).
Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit).
No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
- Entrez GeneID:
- 1633
- Protein Accession#:
- NP_000779;P27707
- Gene Name:
- DCK
- Gene Alias:
- MGC117410,MGC138632
- Gene Description:
- deoxycytidine kinase
- Omim ID:
- 125450
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq
- Other Designations:
- -
- Gene Pathway