Overview

Product name
Anti-CD45RO antibody [UCHL1] (FITC)

See all CD45RO products (9) ...


Description
Mouse monoclonal [UCHL1] to CD45RO (FITC)


Conjugation
FITC


Conjugation notes
The antibody is conjugated with fluorescein isothiocyanate isomer 1 (FITC). The molecular F/P ratio is between 5 and 10.


Specificity
ab51564 is directed against the CD45RO antigen (restricted T200, gp180), a component of the Leukocyte Common Antigen (LCA) complex, which is expressed on human T lymphocytes (40% of CD4 and 20% of CD8 positive T lymphocytes), most thymocytes and the majority of T cell malignancies. ab51564 also reacts weak with most human granulocytes and monocytes, and reacts hardly with B lymphocytes, NK cells or thrombocytes.


Tested applications
Flow Cyt, ICC/IFmore details


Cross reactivity
 
Reacts with
Human
 


Immunogen


T cell line CA1 (Human)




Positive control
CD45RO positive cells in peripheral blood.

Properties

Form
Liquid


Storage instructions
Store at +4°C in the dark. Do not freeze.


Storage buffer
Preservative: 0.1% Sodium Azide

Constituents: 1% BSA, PBS


Concentration

 


    
100tests at mg/ml



Purity
Ion Exchange Chromatography


Purification notes
The antibody was purified from ascites using column chromatography


Primary antibody notes
This antibody would be useful for enumeration of CD45RO positive cells in peripheral blood.


Clonality
Monoclonal


Clone number
UCHL1


Myeloma
NS1


Isotype
IgG2a

http://www.abcam.com/CD45RO-antibody-UCHL1-FITC-ab51564.html