ARFL1,ADP核糖基化样因子1抗体

产品编号HZ-12522R
英文名称ARFL1
中文名称ADP核糖基化样因子1抗体
别 名ADP ribosylation factor like 1; ADP-ribosylation factor-like protein 1; ARFL1; Arl1; ARL1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导 转运蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Pig, Rabbit, Sheep,
ARFL1,ADP核糖基化样因子1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量20kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ARFL1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ARFL1,ADP核糖基化样因子1抗体PubMedPubMed
产品介绍background:
The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins (1–3). ARFs influence vesicle trafficking and signal transduction in eukaryotic cells (1–3). ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes (3–7). Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospholipase D (5–7). The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization (8,9). The human ARF6 gene maps to chromosome 7q22.1, contains five exons and four introns, and encodes a 175 amino acid protein (8,9).

Function:
GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.

Subunit:
The GTP-bound form interacts with GOLGA1 (By similarity). The GTP-bound form interacts with GOLGA4 and RGPD8. The GTP-bound form directly interacts with ARFIP2; this interaction leads to a > 4-fold increase in the amount of bound GTP at steady state level. Binds to SCOC, preferentially in its GTP-bound form. May interact with UNC119.

Subcellular Location:
Golgi apparatus membrane.

ARFL1,ADP核糖基化样因子1抗体Tissue Specificity:
Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Similarity:
Belongs to the small GTPase superfamily. Arf family.

Database links:
UniProtKB/Swiss-Prot: P40616.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.