SAE1 polyclonal antibody
产品名称： SAE1 polyclonal antibody
英文名称： SAE1 polyclonal antibody
产品编号： PAB16904
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SAE1.
- Immunogen:
- A synthetic peptide corresponding to a region surrounding S185 of human SAE1.
- Host:
- Rabbit
- Reactivity:
- Bovine, Dog, Human, Mouse, Rat
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:5000-1:25000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1.
Lois LM, Lima CD.EMBO J. 2005 Feb 9;24(3):439-51. Epub 2005 Jan 20.
- 2.
- Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT.J Biol Chem. 1999 Apr 9;274(15):10618-24.
- 3.
- Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Gong L, Li B, Millas S, Yeh ET.FEBS Lett. 1999 Apr 1;448(1):185-9.
- Applications
- Western Blot (Cell lysate)
- Western blot using SAE1 polyclonal antibody (Cat # PAB16904) shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1 : 1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.
Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX.
- Entrez GeneID:
- 10055
- Gene Name:
- SAE1
- Gene Alias:
- AOS1,FLJ3091,HSPC140,SUA1
- Gene Description:
- SUMO1 activating enzyme subunit 1
- Gene Ontology:
- Hyperlink
- Other Designations:
- SUMO-1 activating enzyme E1 N subunit,SUMO-1 activating enzyme subunit 1,activator of SUMO1,sentrin/SUMO-activating protein AOS1,ubiquitin-like protein SUMO-1 activating enzyme
- Gene Pathway