研究领域 细胞生物  神经生物学  干细胞  细胞分化   

抗体来源 Rabbit 

克隆类型 Polyclonal 

交叉反应 Human, Mouse, Rat,  (predicted: Pig, ) 

产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 37kDa 

细胞定位 细胞外基质 分泌型蛋白  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human Wnt3a:281-352/352  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Function:

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Subunit:

Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6.

Subcellular Location:

Secreted, extracellular space, extracellular matr

Tissue Specificity:

Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.

Post-translational modifications:

Palmitoylation at Wnt3a是Wnt家族的成员之一,对神经干细胞的增殖及分化有一定的调控作用,Wnt3a蛋白可以促进神经干细胞向神经元分化.

产品图片

Sample: U2os Cell(Human)Lysate at 30 ug Primary: Anti- Wnt3a (bs-1700R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 37kD Sample: Brain(Rat) lysate at 30ug; Uterus(Mouse ) lysates, 30ug; Primary: Anti-Wnt3a (bs-1700R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution; Predicted band size : 37kD Observed band size : 37kD Images provided the Independent Validation Program (badge number 029652)Formalin-fixed and paraffin embedded human small intestine labeled with Rabbit Anti-Wnt3a Polyclonal Antibody (bs-1700R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt3a Polyclonal Antibody, Unconjugated(bs-1700R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining