Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)
产品名称： Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)
英文名称： Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)
产品编号： 92566ES08
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:09:52
使用范围： null
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Product Information
Product Name
Cat#
Size
Price（¥）
Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)
92566ES08
5 μg
815.00
92566ES60
100 μg
7335.00
92566ES76
500 μg
19055.00
Product Description
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. The Hedgehog (Hh) family of secreted proteins plays an essential role in metazoan development, and defects in the Hh pathway lead to congenital abnormalities such as holoprosencephaly and certain forms of cancer. Shh is the most widely expressed of the many ligands, and much of what is known about vertebrate Hh signaling stems from studies on Shh. Though similar in many ways to the DrosophilaHh pathway, several features of vertebrate Hh signaling make it unique.
Product Properties
Synonyms
HHG1, HHG-1, HLP3, HPE3, MCOPCB5
Accession
GeneID
Source
E.coli-derived human Shh protein, Cly25-Gly197, with an N-terminal Met.
Molecular Weight
Approximately 19.8 kDa.
AA Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 98% by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 103 IU/mg. Fully biologically active when compared to standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220210