TLR-3 is a single-pass type I receptor that binds to and signals the presence of microbial pathogens and double stranded RNA (dsRNA) viruses. Signaling through TLR-3 can promote the NF-κB pathway to initiate innate and adaptive immune responses to bacterial and viral infections, as well as the p53 pathway to trigger apoptosis in cells infected with dsRNA viruses. TLR-3 belongs to a family of structurally-related toll-like receptors (TLRs) containing an N-terminal domain rich in leucine repeats, and a C-terminal intracellular Toll/interleukin (IL)-1 (TIL) domain. TLR-3 is expressed primarily in dendritic cells of the placenta and pancreas where it can reside on both sides of the plasma membrane, and in the endosomal compartment of the cells.
The BioGems Human TLR-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TLR-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TLR-3. It contains NS0-expressed recombinant Human TLR-3. The detection antibody is a biotinylated goat polyclonal antibody specific for TLR-3. The kit is analytically validated with ready to use reagents.