PAK1 (Human) Recombinant Protein
产品名称: PAK1 (Human) Recombinant Protein
英文名称: PAK1 (Human) Recombinant Protein
产品编号: P5788
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Human PAK1 (NP_002567.3, 1 a.a. - 545 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 88
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 84 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000.
Substrate : LIMKtide. ATP: 100 µM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- SDS-PAGE Stained with Coomassie Blue
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 5058
- Protein Accession#:
- NP_002567.3
- Gene Name:
- PAK1
- Gene Alias:
- MGC130000,MGC130001,PAKalpha
- Gene Description:
- p21 protein (Cdc42/Rac)-activated kinase 1
- Omim ID:
- 602590
- Gene Ontology:
- Hyperlink
- Gene Summary:
- PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- STE20 homolog, yeast,p21-activated kinase 1,p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast),p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
- Gene Pathway
- Axon guidance
- Chemokine signaling pathway
- Epithelial cell signaling in Helicobacter pylori infection
- ErbB signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- MAPK signaling pathway
- Natural killer cell mediated cytotoxicity
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- T cell receptor signaling pathway