PRKCA monoclonal antibody (M01), clone 2F11
产品名称： PRKCA monoclonal antibody (M01), clone 2F11
英文名称： PRKCA monoclonal antibody (M01), clone 2F11
产品编号： H00005578-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant PRKCA.
- Immunogen:
- PRKCA (NP_002728, 563 a.a. ~ 672 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- KEAVSICKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- PRKCA monoclonal antibody (M01), clone 2F11 Western Blot analysis of PRKCA expression in HeLa ( Cat # L013V1 ).
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to PRKCA on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged PRKCA is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between FAS and PRKCA HeLa cells were stained with anti-FAS rabbit purified polyclonal 1:1200 and anti-PRKCA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Entrez GeneID:
- 5578
- GeneBank Accession#:
- NM_002737
- Protein Accession#:
- NP_002728
- Gene Name:
- PRKCA
- Gene Alias:
- AAG6,MGC129900,MGC129901,PKC-alpha,PKCA,PRKACA
- Gene Description:
- protein kinase C, alpha
- Omim ID:
- 176960
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
- Other Designations:
- aging-associated gene 6,protein kinase C alpha type
- Gene Pathway
- Calcium signaling pathway
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Leukocyte transendothelial migration
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Natural killer cell mediated cytotoxicity
- Non-small cell lung cancer
- Pathogenic Escherichia coli infection - EHEC
- Pathways in cancer
- Phosphatidylinositol signaling system
- Tight junction
- Vascular smooth muscle contraction
- VEGF signaling pathway
- Vibrio cholerae infection
- Wnt signaling pathway