JNK抗体-第二抗体-抗体-生物在线
上海信裕生物科技有限公司
JNK抗体

JNK抗体

商家询价

产品名称： JNK抗体

英文名称： JNKAntibody

产品编号： XY51151-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 JNK抗体

Applications
In-house applications ELISA, WB, IP, IF     Application not tested? Please see our Guarantee.
Published applications WB Publications 9 citations –view here
Species specificity Human, Mouse; other species not tested.
Published Species Human, mouse, rat
Images
Prev1NextView more validation data
  • 51151-1-AP;SH-SY5Y cellSH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51151-1-AP(JNK antibody) at dilution of 1:300
  • 51151-1-AP;HEK-293 cellsIP Result of anti-JNK (IP:51151-1-AP, 4ug; Detection:51151-1-AP 1:600) with HEK-293 cells lysate 2000ug.
  • 51151-1-AP;SH-SY5Y cellsImmunofluorescent analysis of ( -20℃ Acetone ) fixed SH-SY5Y cells using 51151-1-AP( JNK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in SH-SY5Y cells, A431 cells, HEK-293 cells, HeLa cells, HT-1080 cells, PC-3 cells
Positive IP detected in HEK-293 cells
Positive IF detected in SH-SY5Y cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IF : 1:50-1:500
 JNK抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information JNK抗体
Immunogen Peptide Full name Mitogen-activated protein kinase 8
Calculated molecular
weight		 48 kDa Observed molecular 
weight		 44-48 kDa
GenBank accession 
number		 BC130570 Gene ID (NCBI) 5599
Gene symbol MAPK8 Synonyms C Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1
Background

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). JNK抗体

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