Anti-Human MEC
Catalog Number:
500-P297
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Anti-Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix.
Immunogen:
E.coli derived Recombinant Human MEC (CCL28) (PeproTech catalog #300-57)
Application:
ELISA
Sandwich
To detect hMEC by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MEC (500-P297Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC. 

ELISA: Sandwich
 
Western Blot
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. 

Western Transfer (Western Blot)
 
 
 
 
 
Research Interests:
Chemotaxis
Wound Healing
 
 
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.