GPNMB抗体-抗体-抗体-生物在线
GPNMB抗体

GPNMB抗体

商家询价

产品名称： GPNMB抗体

英文名称： GPNMB

产品编号： 2684

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： WB ELISA IHC-P IHC-F IF

上海雅吉生物科技有限公司
  • 联系人 :
  • 地址 : 上海市闵行区元江路5500号第1幢5658室
  • 邮编 :
  • 所在区域 : 上海
  • 电话 : 158****3937
  • 传真 : 021-34661275
  • 邮箱 : yajikit@163.com
进入展台

 

中文名称 GPNMB抗体
别    名 NMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL; Dchil; ipd; Glycoprotein (transmembrane) nmb; Glycoprotein nmb; HGFIN; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB; Osteoactivin; GPNMB_HUMAN.  

 

研究领域 免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Pig, Cow, Horse, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 61kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GPNMB:81-180/572 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Could be a melanogenic enzyme.

Subcellular Location:
Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:
Not restricted to the melanocytic lineage.

Similarity:
Belongs to the PMEL/NMB family.
Contains 1 PKD domain.

SWISS:
Q14956

Gene ID:
10457

Database links:

Entrez Gene: 10457 Human

Entrez Gene: 93695 Mouse

Entrez Gene: 113955 Rat

Omim: 604368 Human

SwissProt: Q14956 Human

SwissProt: Q99P91 Mouse

SwissProt: Q6P7C7 Rat

Unigene: 190495 Human

Unigene: 302602 Mouse

Unigene: 13778 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample:
PANC-1(Human) Cell Lysate at 30 ug
Primary: Anti-GPNMB (bs-2684R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 101 kD
Sample:
Lane1: Liver(Rat) Lysate at 30 ug
Lane2: Colon carcinoma(Human) Lysate at 30 ug
Primary: Anti-GPNMB/Osteoactivin (bs-2684R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 61kD
Observed band size : 61kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPNMB) Polyclonal Antibody, Unconjugated (bs-2684R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(bs-2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

​