Description

The choroid plexus plays a wide range of roles in brain development, maturation, aging process, endocrine regulation, and pathogenesis of certain neurodegenerative diseases [1]. The choroid plexuses consist of a single layer of epithelial cells enclosing a vascular core that together form the blood-cerebrospinal fluid barrier [2]. The choroidal capillary is a single layer of endothelial cells interrupted by “pores” which exhibit a diaphragm between the lumen and the interstitial space. Study shows that choroid plexus endothelial cells express high Glut1 glucose transporter [3]. The high glucose transport densities in choroids plexus endothelial cells is consistent with the suggestion that choroids epithelial and endothelial cells provide a metabolic work capability for maintaining ionic gradients and secretory functions across the blood-cerebrospinal fluid barriers.

HCPEC from ScienCell Research Laboratories are isolated from human brain. HCPEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCPEC are characterized by immunofluorescent method with antibodies to VWF/Factor VIII, CD31(PCAM) and by uptake of DiI-Ac-LDL. HBMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPEC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HCPEC in vitro.

Product Use

HCPEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Zheng, W. and Zhao, Q. (2002) Establishment and characterization of an immortalized Z310 choroidal epithelial cell line from murine choroids plexus. Brain Res. 958:371-380.
[2] Thomas, S. A., Bye, A. and Segal, M. B. (2001) Transport Characteristics of the Anti-human Immunodeficiency Virus Nucleoside Analog, Abacavir, into Brain and Cerebrospinal Fluid. J. Pharmacol. Exp. Ther. 298(3):947-53.
[3] Cornford, E. M., Hyman, S., Cornford, M. E. and Damian, R. T. (1998) Glut1 glucose transporter in the primate choroids plexus endothelium. J. Neuropathol. Exp. Neurol. 57:404-411.