上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理:
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。
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人脊髓纤维环细胞说明:
货号:4810
Description
Degeneration of the intervertebral disk is a major pathological process implicated in low back pain. The causes of low back pain have been suggested to involve genetic, environmental, and psychosocial factors [1, 2]. The anulus fibrosus makes up the peripheral portion of disk structure, composed of fibrocartilage and type I and II collagen. Cells of the intervertebral disc are influenced by both biophysical and mechanical factors in their local environment. The anulus fibrosus cells can be stimulated by interleukin 1 beta to produce factors implicated in local degradative and inflammatory processes [3]. Although mechanical stress is an important modulator of the degeneration, the underlying molecular mechanism remains unclear. The human annulus firosus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.
HAFC from ScienCell Research Laboratories are isolated from annulus fibrosus of human intervertibral disc. HAFC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAFC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HAFC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAFC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use nucleus pulposus cell medium (NPCM, Cat. No. 4801) for the culturing of HAFC in vitro.
Product Use
HAFC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1]. Pincus T, Burton AK, Vogel S, Field AP: A systematic review of psychological factors as predictors of chronicity/disability in prospective cohorts of low back pain. Spine 2002, 27:109-120
[2]. Rannou F, Corvol MT, Hudry C, Anract P, Dumontier MF, Tsagris L, Revel M, Poiraudeau S (2000) Sensitivity of anulus fibrosus cells to interleukin 1 beta. Comparison with articular chondrocytes. Spine 25(1):17-23.
[3]. Annunen S, Paassilta P, Lohiniva J, Perala M, Pihlajamaa T, Karppinen J, Tervonen O, Kroger H, Lahde S, Vanharanta H, Ryhanen L, Goring HH, Ott J, Prockop DJ, Ala-Kokko L: An allele of COL9A2 associated with intervertebral disc disease. Science 1999, 285:409-412.
12.骨骼细胞系统
4600 人颅骨造骨细胞 (HCO) ( 5×105 )
4700 人滑膜细胞(HS)( 5×105 )
4800 人髓核细胞(HNPC)(5×105)
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