MBD2 polyclonal antibody
产品名称： MBD2 polyclonal antibody
英文名称： MBD2 polyclonal antibody
产品编号： PAB7163
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Goat polyclonal antibody raised against synthetic peptide of MBD2.
- Immunogen:
- A synthetic peptide corresponding to internal region of human MBD2.
- Sequence:
- C-RNDPLNQNKGKPDLN
- Host:
- Goat
- Theoretical MW (kDa):
- 43.3
- Reactivity:
- Human
- Specificity:
- This antibody is expected to recognize isoform 1 (NP_0.03918.1) only.
- Form:
- Liquid
- Purification:
- Antigen affinity purification
- Concentration:
- 0.5 mg/mL
- Storage Buffer:
- In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:64000)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.
Auriol E, Billard LM, Magdinier F, Dante R.Nucleic Acids Res. 2005 Jul 28;33(13):4243-54. Print 2005.
- Applications
- Western Blot (Cell lysate)
- MBD2 polyclonal antibody (Cat # PAB7163) (0.3 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Entrez GeneID:
- 8932
- Protein Accession#:
- NP_0.03918.1
- Gene Name:
- MBD2
- Gene Alias:
- DKFZp586O0821,DMTase,NY-CO-41
- Gene Description:
- methyl-CpG binding domain protein 2
- Omim ID:
- 603547
- Gene Ontology:
- Hyperlink
- Gene Summary:
- DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq
- Other Designations:
- -