REDD1 SPECIFIC抗体Applications
| In-house applications |
ELISA, IP, WB, IHC Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, IP, WB |
Publications |
116 citations –view here |
| Species specificity |
Human, Mouse, Rat; other species not tested. |
| Published Species |
Gerbil, human, Meriones unguiculatus, mouse, pig, rabbit, rat |
|
Images
Prev12Next
 WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells. K-562 cells were subjected to SDS PAGE followed by western blot with 10638-1-AP(REDD1 antibody) at dilution of 1:1000 IP Result of anti-REDD1 (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug. Immunohistochemical of paraffin-embedded human kidney using 10638-1-AP(REDD1 antibody) at dilution of 1:100 (under 40x lens)
|
| Positive WB detected in |
K-562 cells, A549 cells, DU 145 cells, LNCaP cells, MCF7 cells, PC-3 cells, Raji cells |
| Positive IP detected in |
MCF-7 cells |
| Positive IHC detected in |
Human kidney tissue, human breast cancer tissue, human gliomas tissue, human heart tissue, human liver tissue, human lung cancer tissue, human pancreas cancer tissue, human placenta tissue, human spleen tissue, human testis tissue |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:50-1:200 |
REDD1 SPECIFIC抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information REDD1 SPECIFIC抗体
| Immunogen |
REDD1 specific fusion protein ag0965 |
Full name |
DNA-damage-inducible transcript 4 |
Calculated molecular
weight |
25 kDa |
Observed molecular
weight |
35 kDa |
GenBank accession
number |
BC007714 |
Gene ID (NCBI) |
54541 |
| Gene symbol |
DDIT4 |
Synonyms |
DDIT4, Dig2, FLJ20500, REDD 1, REDD1, RP11 442H21.1, RTP801 |
Background
|
REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific ti the REDD1 from siRNA experiment (PMID:24713927) REDD1 SPECIFIC抗体
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